Eicosanoids are bioactive lipids derived from arachidonic acid. Metabolites of other polyunsaturated fatty acids are, together with the eicosanoids, collectively known as oxylipins (including the pro-resolving mediators). They are produced mainly via three enzymatic pathways: cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP), as well as through auto-oxidation. Among the COX and LOX metabolites, prostaglandins and leukotriens are found. CYP metabolites include fatty acid epoxides and downstream diols. Other related fatty acid metabolites include endocannabinoids such as N-arachidonoylethanolamine (AEA or anandamide) and 2-arachidonoylglycerol (2-AG), as well as other fatty acid amides and glycerol esters.

GENERAL INFORMATION
NUMBER OF SAMPLES: 30-80 samples (analysis of fewer or more samples has to be discussed with SMC). MATRICES: Plasma, serum, cell cultures (other matrices have to be communicated with SMC).

INCLUDED IN THE EICOSANOID ANALYSIS

  • Sample preparation (including solid phase extraction)
  • Analysis by LC-QqQ-MSMS
  • Quality assurance of the analysis
  • Quantification of eicosanoids (extended panel of oxylipins, with or without endocannabinoids profiling can be discussed)
  • Initial statistical evaluation of the generated data according to the question asked in the project (multivariate and univariate data analysis)
  • Report and feedback
  • Archiving of raw data
  • SMC will not be listed as co-authors on a future publication but will be acknowledged


REFERENCES
Gouveia-Figueira S, Späth J, Zivkovic AM, Nording ML. (2015) Profiling the oxylipin and endocannabinoid metabolome by UPLC-ESI-MS/MS in human plasma to monitor postprandial inflammation. PLoS ONE 10(7): e0132042.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506044/

Gouveia-Figueira S, Nording ML. (2015) Validation of a tandem mass spectrometry method using combined extraction of 42 oxylipins and 15 endocannabinoid-related compounds including prostamides from biological matrices. Prostaglandins Other Lipid Mediat. 121:110-121.
http://www.sciencedirect.com/science/article/pii/S109888231500074X

Gouveia-Figueira S, Nording ML. (2014) Development and validation of a sensitive UPLC-ESI-MS/MS method for the simultaneous quantification of 15 endocannabinoids and related compounds in milk and other biofluids. Anal Chem 86(2):1186-1195.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109781/

Contact

Jonas Gullberg
Head of Facility
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