Eicosanoids by LC-QqQ-MSMS

Eicosanoids are bioactive lipids derived from arachidonic acid. Metabolites of other polyunsaturated fatty acids are, together with the eicosanoids, collectively known as oxylipins (including the pro-resolving mediators). They are produced mainly via three enzymatic pathways: cyclooxygenase (COX), lipoxygenase (LOX), and cytochrome P450 (CYP), as well as through auto-oxidation. Among the COX and LOX metabolites, prostaglandins and leukotriens are found. CYP metabolites include fatty acid epoxides and downstream diols. Other related fatty acid metabolites include endocannabinoids such as N-arachidonoylethanolamine (AEA or anandamide) and 2-arachidonoylglycerol (2-AG), as well as other fatty acid amides and glycerol esters.

GENERAL INFORMATION
NUMBER OF SAMPLES: 30-80 samples (analysis of fewer or more samples has to be discussed with SMC).
MATRICES: Plasma, serum, cell cultures (other matrices have to be communicated with SMC).

SMC is an independent partner and will be mentioned in the acknowledgements if the data is published

INCLUDED IN THE EICOSANOID ANALYSIS

  • Sample preparation (including solid phase extraction)
  • Analysis by LC-QqQ-MSMS
  • Quality assurance of the analysis
  • Quantification of eicosanoids (extended panel of oxylipins, with or without endocannabinoids profiling can be discussed)
  • Initial statistical evaluation of the generated data according to the question asked in the project (multivariate and univariate data analysis)
  • Report and feedback
  • Archiving of raw data


REFERENCES
Gouveia-Figueira S, Späth J, Zivkovic AM, Nording ML. (2015) Profiling the oxylipin and endocannabinoid metabolome by UPLC-ESI-MS/MS in human plasma to monitor postprandial inflammation. PLoS ONE 10(7): e0132042.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506044/

Gouveia-Figueira S, Nording ML. (2015) Validation of a tandem mass spectrometry method using combined extraction of 42 oxylipins and 15 endocannabinoid-related compounds including prostamides from biological matrices. Prostaglandins Other Lipid Mediat. 121:110-121.
http://www.sciencedirect.com/science/article/pii/S109888231500074X

Gouveia-Figueira S, Nording ML. (2014) Development and validation of a sensitive UPLC-ESI-MS/MS method for the simultaneous quantification of 15 endocannabinoids and related compounds in milk and other biofluids. Anal Chem 86(2):1186-1195.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109781/