TMAO is an amine oxide that can be quantified using UHPLC-QqQ-MSMS. Quantification of Choline and Betaine is included in the standard procedure as well.
See this example.
GENERAL INFORMATION
NUMBER OF SAMPLES: 50-200 samples (analysis of fewer or more samples has to be discussed with SMC).
MATRICES: Plasma, serum, CSF, and cell cultures (other matrices have to be communicated with SMC).
SMC is an independent partner and will be mentioned in the acknowledgements if the data is published.
INCLUDED IN THE TMAO ANALYSIS
- Sample preparation (e.g. methanol/water extraction)
- Analysis by LC-QqQ-MSMS
- Quality assurance of the analysis
- Quantification of TMAO
- Initial statistical evaluation of the generated data according to the question asked in the project (multivariate and univariate data analysis)
- Report and feedback
- Full access to raw data